Journal: Bioactive Materials
Article Title: Fused exosomal targeted therapy in periprosthetic osteolysis through regulation of bone metabolic homeostasis
doi: 10.1016/j.bioactmat.2025.04.006
Figure Lengend Snippet: Regulation of osteoblast differentiation by f-exo in vitro (A, E-F) Osteogenesis of BMSC induced by f-exo in vitro assessed by ARS, sirius red staining, ALP staining, and their quantifications. Scale bar 3 mm, 400 μm (enlarged) (B, D) the intracellular collagen production assessed by immunofluorescence staining of collagen 1α (Col1a) and quantitative analysis of fluorescence intensity. Scale bar, 200 μm (C, G-H) Osteogenic-related protein analyzed by western blotting, and its relative quantifications (The data in the figures represent the averages ± SD, n = 3 samples per group, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).
Article Snippet: DMEM medium(CYTOCH, Shanghai, China), RPMI 1640medium (BioChannel Biological Technology Co., Ltd.); Fetal Bovine serum (Gibco, USA); Exosome-depleted Fetal Bovine serum (Umibio (Shanghai) Co., Ltd.); Penicillin-Streptomycin Liquid (BDBIO HangZhou China); Cell Freezing Medium (A401; Witcel, Shanghai, China); Trypsin(HAKATA); PBS, TBST (ABclonal Technology, WuHan, China); Cell Plasma Membrane Staining Kit with DiI, DiO, Reactive Oxygen Species assay kit, 4 %PFA, Alkaline Phophatase Stain Kit (Beyotime, China); Complete Medium For BMSC Culture Osteogenic Differentiation (OriCell®, from Cyagen Biosciences(Guangzhou)Inc.); Goat Serum Albumin (Biodragon, AbBox, China); TRAP Activity Assay, KitModified Sirius Red Stain Kit (Beijing Solarbio Science & Technology Co., Ltd.); FITC Phalloidin, DiR Iodide (Yeasen Biotechnology (Shanghai) Co., Ltd.); BCA Protein Quantification Kit (Smar-lifesciences, SLR01201); SDS-PAGE Loading Buffer (Swiss Affinibody LifeScience AG); Titanium nanopowder (Aladdin, Shanghai); Anti-GAPDH antibody (Abcam, UK), Anti-CD63 antibody (Abcam, UK), Anti-TSG101 antibody (Abcam, UK), Anti-Calnexin antibody (Abcam, UK), Anti-col1a antibody (Abcam, UK), Anti-sp7 antibody (Abcam, UK), Anti-runx2 antibody (Abcam, UK), Anti-CTSK antibody (Abcam, UK), Anti-NFATc-1 antibody (Abcam, UK), Anti-TRAP antibody (Abcam, UK), Anti-EMCN antibody (Proteintech, China), Anti-HIF1α antibody (Abcam, UK), Anti-CD31 antibody (Abcam, UK), Anti-VEGF antibody (Abcam, UK).
Techniques: In Vitro, Staining, Immunofluorescence, Fluorescence, Western Blot
